limonene-3-hydroxylase
S. cerevisiae cds limonene-3-hydroxylaseBBF10K_000243
source
Mentha spicata
(‚àí)-Limonene-3-hydroxylase (L3OH), using O2 and NADPH, catalyzes the allylic hydroxylation of (‚àí)-limonene at the 3 position to (‚àí)-trans-isopiperitenol.
attr.
Scott Pownall
Usage
growth
shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-limonene-3-hydroxylase
plasmid size
3618
insert size
1617
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value