MMSYN1_0536

Ribosome-recycling factor
BBF10K_000255
source
Synthetic bacterium JCVI-Syn3.0

Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.

attr.
Anton Jackson-Smith and Bioe80 class

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-MMSYN1_0536
plasmid size
2606
insert size
549
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

No Value