PpHR5'-NTS
Pichia NTS 3'-Homology RegionBBF10K_000455
source
Pichia (Komagataella pastoris)
for stable genome integration adjacent to Pichia NTS locus.
attr.
Scott Pownall & Isaac Larkin
Usage
growth
shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AAAA … AAGG - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-PpHR3'-NTS
plasmid size
2416
insert size
415
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value