oriT

S. cerevisiae target oriT
BBF10K_000466
source
Pseudomonas aeruginosa

Trans-kingdom DNA transfer. The origin of transfer (oriT) is necessary for transfer of the DNA that contains it from a bacterial host to recipient during bacterial conjugation. The oriT is cis-acting.

attr.
Scott Pownall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
No Value
promoter
No Value
inducer
No Value

cloning

method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - CGAA … GCAA - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-oriT
plasmid size
2913
insert size
912
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
No Value

protocols and instructions

No Value

intellectual property

We are unaware of third-party property rights claims on uses of this item as of we.