oriT
S. cerevisiae target oriTBBF10K_000466
source
Pseudomonas aeruginosa
Trans-kingdom DNA transfer. The origin of transfer (oriT) is necessary for transfer of the DNA that contains it from a bacterial host to recipient during bacterial conjugation. The oriT is cis-acting.
attr.
Scott Pownall
Usage
growth
shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - CGAA … GCAA - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-oriT
plasmid size
2913
insert size
912
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value