pOSIP-KO (KanR, 186)
pOSIP-KO (KanR, 186)BBF10K_000492
source
No Value
Please note that there is a E59G mutation in Integrase 186. The mutation does not affect device function.
attr.
Drew Endy, Keith Shearwin
Usage
growth
shipping strain
DB3.1
growth conditions
Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.
antibiotic
Kanamycin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
Restriction Ligation
enzyme
Multiple Cloning Site
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
TAAACTGCCAGGAATTGGGGATC
reverse primer
GAAACGCAAAAAGGCCATCCGTC
Construct
plasmid name
pOSIP-KO (KanR, 186)
plasmid size
6298
insert size
N/A
origin
pUC
copy number
High Copy
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere