RAD Module G8-C1

RAD Module G8-C1
BBF10K_000495
source
No Value

Used to switch DNA registers from BP to LR state and back from LR to BP state using two different inducers (SR-latch). Expresses Bxb1 integrase only under the control of the pTET promoter inducible by Anhydrotetracycline (AtC). Expresses both Bxb1 integrase and excisionase under the control of the pBAD promoter inducible by Arabinose. Regulated ColE1 ori (50-75 copies); Chloramphenicol resistance.

attr.
Drew Endy

Usage

growth

shipping strain
DH5alpha
growth conditions
37 C, shaking 300 rpm
antibiotic
Chloramphenicol

expression

strain
No Value
promoter
pTET promoter, pBAD promoter
inducer
Anhydrotetracycline (AtC), Arabinose

cloning

method
No Value
enzyme
No Value
overhangs
3' - No ValueNo Value - 5'

sequencing

forward primer
CAT-R
reverse primer
No Value

Construct

plasmid name
RAD Module G8-C1
plasmid size
7485
insert size
No Value
origin
ColE1
copy number
50-75

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere

protocols and instructions

No Value

citations

Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351

intellectual property

We are unaware of third-party property rights claims on uses of this item as of we.