NOR gate-V2.0

NOR gate-V2.0
BBF10K_000499
source
No Value

Two-input recombinase logic NOR gate. Produces GFP only when no recombinase has been expressed and acted on the DNA registers (binary output number: 1000). Consists of a promoter followed by two transcriptors (asymmetric transcriptional terminators flanked by inverted recombination sites) in permissive orientation placed in series. When one or both terminators are inverted, GFP is not expressed anymore. Operated by recombinases Bxb1 and TP901 (e.g. using the dual controller). Low copy ppSC101 ori (5-10 copies)- Ampicilin resistance.

attr.
Drew Endy

Usage

growth

shipping strain
DH5alpha
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
N/A
enzyme
N/A
overhangs
3' - No ValueNo Value - 5'

sequencing

forward primer
No Value
reverse primer
No Value

Construct

plasmid name
NOR gate-V2.0
plasmid size
5030
insert size
No Value
origin
pSC101
copy number
5-10

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere

protocols and instructions

No Value

citations

Amplifying genetic logic gates. Bonnet J, Yin P, Ortiz ME, Subsoontorn P, Endy D. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28. 10.1126/science.1232758 PubMed 23539178

intellectual property

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