CD19CAR-41BBz

anti-CD19 chimeric antigen receptor, intracellular 4-1BBz
BBF10K_000529

source

Human

activating receptor on NK cells to bind to CD19

attr.

James N. Kochenderfer, Wyndham H. Wilson, John E. Janik, Mark E. Dudley, Maryalice Stetler-Stevenson, Steven A. Feldman, Irina Maric, Mark Raffeld, Debbie-Ann N. Nathan, Brock J. Lanier, Richard A. Morgan, and Steven A. Rosenberg

Usage

growth

shipping strain

DH5alpha

growth conditions

37 C, shaking 300 rpm

antibiotic

Kanamycin

expression

strain

Human

promoter

SFFV

inducer

N/A

cloning

method

Gibson assembly into viral backbone

enzyme

BamHI

overhangs

3' - CAGACTGAGTCGCCCGGGGGgatccgccacc … ggatccGCCACCAACTTCAGCCTGCT - 5'

sequencing

forward primer

CAGACTGAGTCGCCCGGGGGgatccgccacc

reverse primer

gcaggctgaagttggtggcggatcc

Construct

plasmid name

pOpen-CD19CAR-41BBz

plasmid size

6024

insert size

1636

origin

oriS

copy number

medium

genbank file

Safety

BSL

other information

No Value

References

primary references

NCBI

N/A

UniProt

N/A

Available Elsewhere

FALSE

protocols and instructions

No Value

citations

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993617/?report=reader

intellectual property

We are unaware of third-party property rights claims on uses of this item as of .