pInducible

pOpen_v4
BBF10K_000589
source
Synthetic

Golden-Gate-compatible cloning vector. Low-copy, includes an indicuble high-copy origin. For cloning difficult parts.

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin

expression

strain
Escherichia coli
promoter
LacOP induces high copy
inducer
IPTG

cloning

method
GoldenGate
enzyme
BbsI, AarI
overhangs
3' - varies … varies - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen_Inducible
plasmid size
6238
insert size
No Value
origin
oriS, ColE1
copy number
low; high

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

citations

No Value

intellectual property

We are unaware of third-party property rights claims on uses of this item.