ispD

2-D-methyl-D-erythritol 4-phosphatecytidylyltransferase
BBF10K_000769
source
Bacillus subtilis 168

4-diphosphocytidyl-2C-methyl-D-erythritol synthase;MEP cytidylyltransferase; MCT; catalyzes the formation of4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and2-C-methyl-D-erythritol 4-phosphate; involved inisoprenoid and isopentenyl-PP biosynthesis; formshomodimers

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ispD
plasmid size
2722
insert size
699
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.