rplJ
50S ribosomal protein L10BBF10K_000774
source
		    Bacillus subtilis 168
                binds the two ribosomal protein L7/L12 dimers andanchors them to the large ribosomal subunit
attr.
		    Keoni Gandall
                Usage
growth
shipping strain
		    {shipping_strain}
                growth conditions
		    37 C, shaking 300 rpm
                antibiotic
		    ampicillin
                expression
strain
		    N/A
                promoter
		    N/A
                inducer
		    N/A
                cloning
method
		    GoldenGate
                enzyme
		    BsaI
                overhangs
		    3' - AATG … GCTT - 5'
                sequencing
forward primer
		    M13 For
                reverse primer
		    M13 Rev
                Construct
plasmid name
			pOpen-rplJ
                    plasmid size
			2524
                    insert size
                        
				501
			
                    origin
			ColE1 High Copy
                    copy number 
			500-700
                    Safety
BSL
			BSL1
                    other information
No Value
References
Available Elsewhere
	    FALSE