rplE

50S ribosomal protein L5
BBF10K_000792
source
Bacillus subtilis 168

part of 50S and 5S/L5/L18/L25 subcomplex; contacts5S rRNA and P site tRNA; forms a bridge to the 30S subunitin the ribosome by binding to S13

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-rplE
plasmid size
2563
insert size
540
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.