rplF

50S ribosomal protein L6
BBF10K_000795
source
Bacillus subtilis 168

ribosomal protein L6 appears to have arisen as aresult of an ancient gene duplication as based onstructural comparison of the Bacillus stearothermophilusprotein; RNA-binding appears to be in the C-terminaldomain; mutations in the L6 gene confer resistance toaminoglycoside antibiotics such as gentamicin and theseoccur in truncations of the C-terminal domain; it has beenlocalized to a region between the base of the L7/L12 stalkand the central protuberance

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-rplF
plasmid size
2560
insert size
540
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.