rpsE

30S ribosomal protein S5
BBF10K_000797
source
Bacillus subtilis 168

located at the back of the 30S subunit body whereit stabilizes the conformation of the head with respect tothe body; contacts S4 and S8; with S4 and S12 plays a rolein translational accuracy; mutations in this gene resultin spectinomycin resistance

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-rpsE
plasmid size
2524
insert size
501
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item as of 2018-07-02.