ddl

D-alanine--D-alanine ligase
BBF10K_000814
source
Bacillus subtilis 168

D-alanine--D-alanine ligase; DdlA; DdlB;cytoplasmic; catalyzes the formation of D-alanyl-D-alaninefrom two D-alanines in peptidoglycan synthesis; there aretwo forms of this enzyme in Escherichia coli

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ddl
plasmid size
3088
insert size
1065
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.