frr

ribosome-recycling factor
BBF10K_000887
source
Bacillus subtilis 168

Rrf; Frr; ribosome-recycling factor; release factor4; RF4; recycles ribosomes upon translation terminationalong with release factor RF-3 and elongation factor EF-G;A GTPase-dependent process results in release of 50S from70S; inhibited by release factor RF-1; essential forviability; structurally similar to tRNAs

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-frr
plasmid size
2578
insert size
558
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.