nrdF

ribonucleoside-diphosphate reductase subunitbeta
BBF10K_000904
source
Bacillus subtilis 168

B2 or R2 protein; type 1b enzyme; catalyzes therate-limiting step in dNTP synthesis; converts nucleotidesto deoxynucleotides; forms a homodimer and then amultimeric complex with NrdE

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-nrdF
plasmid size
3013
insert size
990
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.