engA

GTPase Der
BBF10K_000926
source
Bacillus subtilis 168

EngA; essential Neisserial GTPase; synchronizescellular events by interacting with multiple targets withtandem G-domains; overexpression in Escherichia colisuppresses rrmJ mutation; structural analysis of theThermotoga maritima ortholog shows different nucleotidebinding affinities in the two binding domains

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-engA
plasmid size
3334
insert size
1311
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.