rpsD

30S ribosomal protein S4
BBF10K_000979
source
Bacillus subtilis 168

primary rRNA binding protein; nucleates 30Sassembly; involved in translational accuracy with proteinsS5 and S12; interacts with protein S5; involved inautogeneously regulating ribosomal proteins by binding topseudoknot structures in the polycistronic mRNA; interactswith transcription complex and functions similar toprotein NusA in antitermination

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-rpsD
plasmid size
2623
insert size
603
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.