mrpE
Na(+)/H(+) antiporter subunit EBBF10K_000994
source
		    Bacillus subtilis 168
                Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
attr.
		    Keoni Gandall
                Usage
growth
shipping strain
		    {shipping_strain}
                growth conditions
		    37 C, shaking 300 rpm
                antibiotic
		    ampicillin
                expression
strain
		    N/A
                promoter
		    N/A
                inducer
		    N/A
                cloning
method
		    GoldenGate
                enzyme
		    BsaI
                overhangs
		    3' - AATG … GCTT - 5'
                sequencing
forward primer
		    M13 For
                reverse primer
		    M13 Rev
                Construct
plasmid name
			pOpen-mrpE
                    plasmid size
			2497
                    insert size
                        
				477
			
                    origin
			ColE1 High Copy
                    copy number 
			500-700
                    Safety
BSL
			BSL1
                    other information
No Value
References
Available Elsewhere
	    FALSE