prfA
peptide chain release factor 1BBF10K_001023
source
Bacillus subtilis 168
recognizes the termination signals UAG and UAAduring protein translation a specificity which isdependent on amino acid residues residing in loops of theL-shaped tRNA-like molecule of RF1; this protein issimilar to release factor 2
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-prfA
plasmid size
3094
insert size
1071
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE