tsaEtRNA threonylcarbamoyladenosine biosynthesis protein TsaE
Escherichia coli str. K-12 substr. MG1655
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
Escherichia coli Top10
37 C, shaking 300 rpm
3' - AATG … GCTT - 5'
ColE1 High Copy
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