rplV

50S ribosomal protein L22
BBF10K_001361
source
Mesoplasma florum L1

binds specifically to 23S rRNA during the earlystages of 50S assembly; makes contact with all 6 domainsof the 23S rRNA in the assembled 50S subunit and ribosome;mutations in this gene result in erythromycin resistance;located near peptidyl-transferase center

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-rplV
plasmid size
2402
insert size
339
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

No Value