rpmJ

50S ribosomal protein L36
BBF10K_001380
source
Mesoplasma florum L1

smallest protein in the large subunit; similar towhat is found with protein L31 and L33 several bacterialgenomes contain paralogs which may be regulated by zinc;the protein from Thermus thermophilus has a zinc-bindingmotif and contains a bound zinc ion; the proteins in thisgroup have the motif

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-rpmJ
plasmid size
2301
insert size
114
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

No Value