ruvB

Holliday junction DNA helicase RuvB
BBF10K_001655
source
Mesoplasma florum L1

promotes strand exchange during homologousrecombination; RuvAB complex promotes branch migration;RuvABC complex scans the DNA during branch migration andresolves Holliday junctions at consensus sequences; formshexameric rings around opposite DNA arms; requires ATP forbranch migration and orientation of RuvAB complexdetermines direction of migration

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ruvB
plasmid size
3011
insert size
948
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.