Mfl541

16S rRNA processing protein
BBF10K_001774
source
Mesoplasma florum L1

An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Mfl541
plasmid size
2564
insert size
501
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.