rpoA
DNA-directed RNA polymerase subunit alphaBBF10K_002611
source
Mycoplasma pneumoniae M129
catalyzes the transcription of DNA into RNA usingthe four ribonucleoside triphosphates as substrates.Dimerization of the alpha subunit is the first step in thesequential assembly of subunits to form the holoenzyme
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-rpoA
plasmid size
3046
insert size
984
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE