nrdF
ribonucleotide-diphosphate reductase subunitbetaBBF10K_002749
source
Mycoplasma pneumoniae M129
B2 or R2 protein; type 1b enzyme; catalyzes therate-limiting step in dNTP synthesis; converts nucleotidesto deoxynucleotides; forms a homodimer and then amultimeric complex with NrdE
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-nrdF
plasmid size
3082
insert size
1020
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE