araD

L-ribulose-5-phosphate 4-epimerase
BBF10K_002923
source
Mycoplasma pneumoniae M129

catalyzes the isomerization of L-ribulose5-phosphate to D-xylulose 5-phosphate in the anaerobiccatabolism of L-ascorbate; links the arabinose metabolicpathway to the pentose phosphate pathway and allows thebacteria to use arabinose as an energy source

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-araD
plasmid size
2791
insert size
729
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.