araD
L-ribulose-5-phosphate 4-epimeraseBBF10K_002923
source
Mycoplasma pneumoniae M129
catalyzes the isomerization of L-ribulose5-phosphate to D-xylulose 5-phosphate in the anaerobiccatabolism of L-ascorbate; links the arabinose metabolicpathway to the pentose phosphate pathway and allows thebacteria to use arabinose as an energy source
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-araD
plasmid size
2791
insert size
729
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE