ygl3

Putative tRNA (cytidine(34)-2'-O)-methyltransferase
BBF10K_002946
source
Mycoplasma pneumoniae M129

Could methylate the ribose at the nucleotide 34 wobble position in tRNA.

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ygl3
plasmid size
2563
insert size
501
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

No Value