9N7polA

9°N-7 DNA polymerase
BBF10K_003247
source
Thermococcus sp. 9°N-7

thermophilic DNA polymerase; genetically engineered to have a decreased 3'→ 5' proofreading exonuclease activity (1-5% of the wildtype). Features a half-life of 6.7 hours at 95°C.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-9N7polA
plasmid size
4451
insert size
2328
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Southworth, M.W., Kong, H., Kucera, R.B., Ware, J., Jannasch, H.W. and Perler, F.B., 1996. Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5'exonuclease activity. Proceedings of the National Academy of Sciences, 93(11), pp.5281-5285. https://doi.org/10.1073/pnas.93.11.5281

intellectual property

We are unaware of third-party property rights claims on uses of this item.