K12polLFDNA Polymerase I, Large (Klenow) Fragment
Escherichia coli (strain K12)
DNA polymerase fragment that retains 5' → 3' polymerase activity and 3’ → 5’ exonuclease activity for removal of precoding nucleotides and proofreading, but loses 5' → 3' exonuclease activity. Usable for synthesis of double-stranded DNA from single-stranded templates, filling in of receded 3' ends of DNA fragments to make 5' overhang blunt, digesting away protruding 3' overhangs, preparation of radioactive DNA probes.
Chiara Gandini, Open Bioeconomy Lab
37 C, shaking 300 rpm
3' - AATG … GCTT - 5'
ColE1 High Copy
other informationNo Value