KODpol

KOD DNA polymerase
BBF10K_003252
source
Thermococcus kodakarensis (Pyrococcus sp. KOD1)

High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. Generates blunt-ended PCR product. Lacks 3'-5' exonuclease activity

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-KODpol
plasmid size
4451
insert size
2328
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Takagi, M., Nishioka, M., Kakihara, H., Kitabayashi, M., Inoue, H., Kawakami, B., Oka, M. and Imanaka, T., 1997. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR. Applied and environmental microbiology, 63(11), pp.4504-4510. https://aem.asm.org/content/63/11/4504.short

intellectual property

We are unaware of third-party property rights claims on uses of this item.