Pwopol

Pwo DNA Polymerase
BBF10K_003254
source
Pyrococcus woesei

DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Pwopol
plasmid size
4451
insert size
2328
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Frey, B. and Suppmann, B., 1995. Demonstration of the Expand PCR system’s greater fidelity and higher yields with a lacI-based PCR fidelity assay. Biochemica, 2, pp.8-9. Ghasemi, A., Salmanian, A.H., Sadeghifard, N., Salarian, A.A. and Gholi, M.K., 2011. Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei. Iranian journal of microbiology, 3(3), p.118.

intellectual property

We are unaware of third-party property rights claims on uses of this item.