THEAQpolA

Taq DNA Polymerase
BBF10K_003257
source
Thermus aquaticus

DNA polymerase used in (routine) PCR method because of its high thermostability, standard testing, screening and output testing. Has a relatively high error rate because it lacks 3'-5' exonuclease activity and proofreading functionality. 5'→3' flap endonuclease degrades displaced strand."

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-THEAQpolA
plasmid size
4622
insert size
2499
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Chien A, Edgar DB, Trela JM (1976). "Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus". J. Bacteriol. 127 (3): 1550–7. PMC 232952 . PMID 8432

intellectual property

We are unaware of third-party property rights claims on uses of this item.