Tlipol (exo)

Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase)
BBF10K_003259
source
Thermococcus litoralis

DNA polymerase; lacks 3' to 5' exonuclease activity. Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the 'homing site' in gene that lack the intein.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Tlipol (exo)
plasmid size
4448
insert size
2325
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Kong, H., Kucera, R.B. and Jack, W.E., 1993. Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. Journal of Biological Chemistry, 268(3), pp.1965-1975.

intellectual property

We are unaware of third-party property rights claims on uses of this item.