TthpolA

Tth DNA polymerase
BBF10K_003260
source
Thermus thermophilus

DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 °C); can therefore be used for PCR amplification of DNA fragments. Also accepts modified deoxyribonucleotides as substrates and can thus be used to label DNA fragments with radiolabeled nucleotides, digoxigenin, or biotin. Intrinsic Mn-dependent RT activity allows the enzyme to efficiently transcribe RNA targets into cDNA. Tth DNA Polymerase has been used for real time PCR.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-TthpolA
plasmid size
4628
insert size
2505
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

RÜTTIMANN, C., COTORÁS, M., ZALDÍÅ, J. and VICUÑA, R., 1985. DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB‐8. European journal of biochemistry, 149(1), pp.41-46.https://doi.org/10.1111/j.1432-1033.1985.tb08890.x Myers, T.W. and Gelfand, D.H., 1991. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry, 30(31), pp.7661-7666. DOI: 10.1021/bi00245a001 Auer, T., Landre, P.A. and Myers, T.W., 1995. Properties of the 5' - 3'Exonuclease/Ribonuclease H Activity of Thermus thermophilus DNA Polymerase. Biochemistry, 34(15), pp.4994-5002. DOI: 10.1021/bi00015a010

intellectual property

We are unaware of third-party property rights claims on uses of this item.