Bstpol

Bst DNA Polymerase, Full Length
BBF10K_003261
source
Bacillus stearothermophilus

full length DNA polymerase from Bacillus stearothermophilus. Has 5' → 3' polymerase and double-strand specific 5' → 3' exonuclease activity, but lacks the 3' → 5' exonuclease activity. Used for isothermal DNA amplification, primer extension, etc.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Bstpol
plasmid size
4754
insert size
2631
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Stenesh, J. and Roe, B.A., 1972. DNA polymerase from mesophilic and thermophilic bacteria: I. Purification and properties of DNA polymerase from Bacillus licheniformis and Bacillus stearothermophilus. Biochimica et Biophysica Acta (BBA)-Nucleic Acids and Protein Synthesis, 272(2), pp.156-166. Kaboev, O.K., Luchkina, L.A., Akhmedov, A.T. and Bekker, M.L., 1981. Purification and properties of deoxyribonucleic acid polymerase from Bacillus stearothermophilus. Journal of bacteriology, 145(1), pp.21-26.

intellectual property

We are unaware of third-party property rights claims on uses of this item.