BsupolLF

Bsu DNA Polymerase I, Large Fragment
BBF10K_003263
source
Bacillus subtilis (strain 168)

Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillus subtilis DNA polymerase I (1), but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis (2)

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-BsupolLF
plasmid size
4181
insert size
2058
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Okazaki, T. and Kornberg, A., 1964. Enzymatic synthesis of deoxyribonucleic acid XV. Purification and properties of a polymerase from Bacillus subtilis. Journal of Biological Chemistry, 239(1), pp.259-268.

intellectual property

We are unaware of third-party property rights claims on uses of this item.