9N7polA (CT)

9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol)
BBF10K_003265
source
Thermococcus sp. 9°N-7

9°N™ DNA polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-9N7polA (CT)
plasmid size
4451
insert size
2328
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Gardner, A.F. and Jack, W.E., 2002. Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases. Nucleic Acids Research, 30(2), pp.605-613. https://doi.org/10.1093/nar/30.2.605

intellectual property

We are unaware of third-party property rights claims on uses of this item.