dbh

Sulfolobus DNA Polymerase IV
BBF10K_003266
source
Sulfolobus solfataricus

Thermostable Y-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions; can be used for DNA repair. Poorly processive and error-prone. Involved in untargeted mutagenesis; copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-dbh
plasmid size
3182
insert size
1059
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Pisani, F. M., Martino, C. D., & Rossi, M. (1992). A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases. Nucleic acids research, 20(11), 2711-2716. Boudsocq, F., Iwai, S., Hanaoka, F. and Woodgate, R., 2001. Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic polη. Nucleic acids research, 29(22), pp.4607-4616. https://doi.org/10.1093/nar/29.22.4607

intellectual property

We are unaware of third-party property rights claims on uses of this item.