T5gene122

T5 DNA Polymerase
BBF10K_003269
source
Escherichia phage T5 (Bacteriophage T5)

Replicates the viral genomic DNA. Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction for proofreading purposes. The DNA synthesis very likely occurs by strand displacement.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T5gene122
plasmid size
4691
insert size
2568
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Chatterjeea, D. K., Fujimurab, R. K., Campbella, J. H., & Gerarda, G. F. (1991). Cloning and overexpression of the gene encoding bacteriophage T5 DNA polymerase. Gene, 97(1), 13-19.

intellectual property

We are unaware of third-party property rights claims on uses of this item.