ECOligA

E. coli DNA Ligase
BBF10K_003271
source
Escherichia coli (strain K12)

Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ECOligA
plasmid size
4139
insert size
2016
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Ishino, Yoshizumi, et al. "Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli." Molecular and General Genetics MGG 204.1 (1986): 1-7.

intellectual property

We are unaware of third-party property rights claims on uses of this item.