HSlig4

DNA Ligase IV
BBF10K_003273
source
Homo sapiens

Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction. Also involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-HSlig4
plasmid size
4658
insert size
2535
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Wei, Y. F., Robins, P., Carter, K., Caldecott, K., Pappin, D. J., Yu, G. L., ... & Schär, P. R. I. M. O. (1995). Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination. Molecular and Cellular Biology, 15(6), 3206-3216.

intellectual property

We are unaware of third-party property rights claims on uses of this item.