THEAQlig

Taq DNA Ligase
BBF10K_003276
source
Thermus aquaticus

Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-THEAQlig
plasmid size
4148
insert size
2025
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Takahashi, Miho, Eiichi Yamaguchi, and Tsuneko Uchida. "Thermophilic DNA ligase. Purification and properties of the enzyme from Thermus thermophilus HB8." Journal of Biological Chemistry 259.16 (1984): 10041-10047.

intellectual property

We are unaware of third-party property rights claims on uses of this item.