RNAse H

RNAse H
BBF10K_003280
source
Escherichia coli (strain K12)

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus. Along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis, and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-RNAse H
plasmid size
2591
insert size
468
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Kanaya, Shigenori, and Robert J. Crouch. "DNA sequence of the gene coding for Escherichia coli ribonuclease H." Journal of Biological Chemistry 258.2 (1983): 1276-1281.

intellectual property

We are unaware of third-party property rights claims on uses of this item.