EcoRIM

EcoRI methyltransferase
BBF10K_003282
source
Escherichia coli (strain?)

Methylase that recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-EcoRIM
plasmid size
3104
insert size
981
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Woodbury, C. P., R. L. Downey, and P. H. Von Hippel. "DNA site recognition and overmethylation by the Eco RI methylase." Journal of Biological Chemistry 255.23 (1980): 11526-11533.

intellectual property

We are unaware of third-party property rights claims on uses of this item.