T3RNAp

T3 RNA Polymerase
BBF10K_003289
source
Enterobacteria phage T3 (Bacteriophage T3)

DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T3gene1
plasmid size
4778
insert size
2655
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Morris, C. E., Klement, J. F., & McAllister, W. T. (1986). Cloning and expression of the bacteriophage T3 RNA polymerase gene. Gene, 41(2), 193-200.

intellectual property

We are unaware of third-party property rights claims on uses of this item.