SP6RNAp

SP6 RNA Polymerase
BBF10K_003291
source
Enterobacteria phage SP6 (bacteriophage SP6

DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-SP6rpol
plasmid size
4748
insert size
2625
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Kotani, H., Ishizaki, Y., Hiraoka, N., & Obayashi, A. (1987). Nucleotide sequence and expression of the cloned gene of bacteriphage SP6 RNA polymerase. Nucleic acids research, 15(6), 2653-2664.

intellectual property

We are unaware of third-party property rights claims on uses of this item.