T5gene12/13

T5 Exonuclease
BBF10K_003313
source
Escherichia phage T5 (Bacteriophage T5)

Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction. Ideal for removal of incomplete ligation products from ligated circular double-stranded DNA, degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning, and degradation of contaminating linear and nicked DNA in plasmid samples

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T5gene12/13
plasmid size
2999
insert size
897
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.